Journal: EBioMedicine

Article Title: The Notch ligand DNER regulates macrophage IFNγ release in chronic obstructive pulmonary disease

doi: 10.1016/j.ebiom.2019.03.054

Figure Lengend Snippet: Nuclear translocation of Notch1 and NFκB active subunits was abrogated in LPS treated BMDM from DNER deficient mice. (a) Representative Western blot of NICD1 protein levels in cytoplasmic and nuclear extracts of samples from WT and DNER deficient BMDM treated with LPS (1μg/ml) for 24 h and untreated (2 independent experiments, 2/3 biological replicates). (b) mRNA abundance of Notch receptors ( Notch1, Notch2 ) and ligands ( Delta4, Jag1 ) in the samples from (a). Two-way ANOVA, Tukey's multiple comparisons test, * P < 0.05, *** P < 0.001. Data shown mean values ± SD. (c) Cytoplasmic and nuclear RelB, c-Rel, p105, p50 and p65 from samples described in (a). (d) Representative Western blots of phosphorylated (p)-IKKα/β, IKKα and IKKβ from 15 min LPS (1μg/ml) treated WT and DNER deficient BMDM. 2 independent experiments and 2/3 biological replicates, n = 3 (e) Enrichment plot of NFkB signalling (GO:0051059) following GSEA analysis of the microarray data from WT M1 vs DNER deficient M1 BMDM.

Article Snippet: Primary antibodies: NICD1 (1:500, ab8925, Abcam), RelB (1:200, sc-226, Santa Cruz), cRel (1:100, sc-6955, Santa Cruz), p105/50 (1:100, sc-1190, Santa Cruz), p65 (1:500, sc-372, Santa Cruz), pIKKα/β (1:1000 #2697, Cell Signalling), IKKα (1:1000 #2682S, Cell Signalling), IKKβ (1:1000 clone D30C6, #8943, Cell Signalling).

Techniques: Translocation Assay, Western Blot, Microarray

Journal: Cell

Article Title: Gene Regulatory Programs Conferring Phenotypic Identities to Human NK Cells

doi: 10.1016/j.cell.2018.11.045

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: DX5 microbeads , Miltenyi , Cat#130–052-501.

Techniques: Microscopy, Recombinant, DNA Library Preparation, Isolation, Microarray, Knock-Out, Expressing, Software

Journal: Biomedical Reports

Article Title: Lipolysis by downregulating miR-92a activates the Wnt/β-catenin signaling pathway in hypoxic rats

doi: 10.3892/br.2020.1340

Figure Lengend Snippet: Analysis of miR-92a expression in the epididymal fat of hypoxic rats. (A) B3L, B6L and B9L were from normoxic rats. (B) D2L, D6L, and D9L were from hypoxic rats. (C) Relative expression of miRNAs in the normoxic and hypoxic rats. miR-92a expression levels in the hypoxic rats were reduced compared with the normoxic rats based on the miRNA microarray analysis. miR, microRNA; Rno, Rattus norvegicus.

Article Snippet: The following reagents and instruments were used: miRcute miRNA Isolation kit (Tiangen Biotech Co., Ltd.), miRcute miRNA cDNA First Strand Synthesis kit (Tiangen Biotech Co., Ltd.), miRcute MiRNA Quantitative Fluorescence Detection kit (cat. no. FP401; Tiangen Biotech Co., Ltd.), SuperReal PreMix SYBR-Green (cat. no. FP204; Tiangen Biotech Co., Ltd.), TIANScript II cDNA First Strand Synthesis kit (cat. no. KR107; Tiangen Biotech Co., Ltd.) and RT-qPCR amplifier (BIOER FQD-96A; Hangzhou Bioer Co., Ltd.).

Techniques: Expressing, Microarray

Journal: Biomedical Reports

Article Title: Lipolysis by downregulating miR-92a activates the Wnt/β-catenin signaling pathway in hypoxic rats

doi: 10.3892/br.2020.1340

Figure Lengend Snippet: Analysis of miR-92a binding with Fzd10. (A) Wild-type and mutant-type binding sequences of miR-92a with Fzd10. (B) Dual luciferase reporter assay. Wild-type miR-92a bound and degraded wild-type Fzd10 mRNA in the Fzd10+miR92a group. Single Fzd10 or mutated Fzd10 mRNA did not result in altered fluorescence values of the empty carrier pRL-TK, and miR-92a was not significantly altered the fluorescence values of the mutated Fzd10 mRNA in the mFzd10+miR92a group. miR/miRNA, microRNA; Fzd10, frizzled 10; mFzd10, mutated Fzd10.

Article Snippet: The following reagents and instruments were used: miRcute miRNA Isolation kit (Tiangen Biotech Co., Ltd.), miRcute miRNA cDNA First Strand Synthesis kit (Tiangen Biotech Co., Ltd.), miRcute MiRNA Quantitative Fluorescence Detection kit (cat. no. FP401; Tiangen Biotech Co., Ltd.), SuperReal PreMix SYBR-Green (cat. no. FP204; Tiangen Biotech Co., Ltd.), TIANScript II cDNA First Strand Synthesis kit (cat. no. KR107; Tiangen Biotech Co., Ltd.) and RT-qPCR amplifier (BIOER FQD-96A; Hangzhou Bioer Co., Ltd.).

Techniques: Binding Assay, Mutagenesis, Luciferase, Reporter Assay, Fluorescence